基于DNA链置换构建逻辑计算模型-《计算机技术与发展》

文章信息/Info

Title:: Construction of Logical Calculation Model Based on DNAStrand Displacement Reaction

作者:: 吴涛; 郭冰洁; 董亚非; 陕西师范大学计算机科学学院,陕西西安,710119;陕西师范大学生命科学学院,陕西西安,710119;陕西师范大学计算机科学学院,陕西西安 710119;陕西师范大学生命科学学院,陕西西安 710119

Author(s):: WU Tao1; GUO Bing-jie2; DONG Ya-fei1; 2; 1. School of Computer Science,Shaanxi Normal University,Xi’an 710119,China;2. School of Life Science,Shaanxi Normal University,Xi’an 710119,China

Keywords:: DNA; NAND gate; half-adder; strand displacement reaction; fluorescent marker

摘要:: 近年来,随着电子元器件的微型化趋势,其传统制作工艺所面临的挑战也日益显著,寻找新型手段辅助甚至替代传统硅基计算机已逐渐成为科学家们的研究重点.所以,随着生物化学技术的不断发展,以DNA分子作为存储数据和运算媒介的新型计算模型引起了研究者的广泛关注.为此,提出了基于DNA链置换与荧光标记的分子逻辑计算模型.该方法利用重金属离子Hg2+能够引发组成DNA分子中胸腺嘧啶(thymine,T)形成T-Hg2+-T错配的形式,通过检测荧光信号强度的相对变化,构建了与非逻辑门(NAND).之后,在前者的基础上利用DNA富含鸟嘌呤(guanine,G)的片段可以形成G-四链体结构的情况下,通过结合氯化血红素(Hemin)形成具有过氧化物酶的活性进而促进过氧化氢(H2 O2)氧化四甲基联苯胺(TMB)得到蓝色的TMB+,以此构建半加器.

Abstract:: In recent years,with the tendency of miniaturization of electronic components,the challenges faced by traditional manufacturingprocess have become more and more obvious. It is becoming more and more important for scientists to find new ways to supplement oreven replace traditional silicon-based computers. Therefore,with the continuous development of biochemical technology,DNA moleculesas a new computational model for storing data and computing media have drawn much attention from researchers. For this reason,wepropose a molecular logic calculation model based on DNA strand displacement and fluorescent labeling. The method uses heavy metal ions Hg2＋ to induce the formation of T-Hg 2＋ -T mismatch in thymine (T) of DNA molecules. By detecting the relative change of fluorescence signal intensity,a NAND gate is constructed. Then,using the guanine (G) -containing fragment of DNA on the basis of the former to form a G-quadruplex structure,a peroxidase-forming activity is formed by binding hemin. Half-adder is constructed by promoting hydrogen peroxide (H2O2 ) tetramethyl benzidine (TMB) to give blue TMB＋.